Analysis of critical parameters in AAV vector production can present bottlenecks that dramatically slow down overall production time. Automated analytical tools with the capacity to perform comprehensive analysis of critical parameters in AAV vector production within a short time frame can overcome this challenge. This case study illustrates how this automated approach allows researchers to evaluate four in-process steps across two independent AAV vector productions in under six hours, measuring total capsid titer and three process-related impurities, with nearly zero hands-on time required to complete a full assessment within a single workday.
Learning Objectives:
Understand how the automated microfluidic platform enables comprehensive analysis of AAV vector production parameters while minimizing sample volume requirements.
Identify the time-saving advantages of conducting multiple analytical assessments in under six hours compared to traditional methods.
Evaluate how the walk-away automation capability reduces hands-on time while maintaining data quality during AAV vector characterization.
Apply insights from the case study to optimize workflow efficiency when measuring both total capsid titer and process-related impurities across multiple production steps.
